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Journal: bioRxiv
Article Title: Granulysin antimicrobial activity promotes dormancy in Mycobacterium tuberculosis
doi: 10.1101/2024.09.27.615427
Figure Lengend Snippet: (a) Schematic overview of the study design. Peripheral blood mononuclear cells (PBMC) from healthy controls (HC) or patients diagnosed with infectious clinical TB (TB) were either exposed to Mycobacterium tuberculosis ( Mtb )-derived stimuli prior flow cytometry analysis (b) or infected with Mtb H37Rv, prior embedding within an extra-cellular matrix for 3D ex-vivo granuloma formation and assessment of the indicated microbiological read-outs (c-d). (b) Dot plot displaying background subtracted frequency of IFNγ-producing CD4 T cells measured by flow cytometry after overnight stimulation with ESAT-6/CFP- 10/TB7.7 peptide pool, Mtb soluble-cell wall proteins (SCWP) or Mtb whole-cell lysate (WCL); lines indicate median. (c) Bacterial load in colony forming units (CFU), eight days post-infection (p.i.). (d) Frequency of dormant-like Mtb based on auramine-O/Nile red dual staining, one day p.i.; bars depict the median. All p values represent Mann-Whitney tests.
Article Snippet: Mtb H37Rv whole cell lysate (NR-14822) and
Techniques: Derivative Assay, Flow Cytometry, Infection, Ex Vivo, Staining, MANN-WHITNEY
Journal: Clinical & Translational Immunology
Article Title: Antibody glycosylation correlates with disease progression in SIV‐ Mycobacterium tuberculosis coinfected cynomolgus macaques
doi: 10.1002/cti2.1474
Figure Lengend Snippet: Simian immunodeficiency virus (SIV) infection impairs humoral immunity. Change from baseline (prior to any infection) in plasma humoral profiles (IgG, IgG1, IgG2, IgG3, FcγRIIa and FcγRIIIa) 8 weeks following infection with Mycobacterium tuberculosis ( Mtb ) in MCM without SIV (naïve; n = 8; yellow left bar) or coinfected with SIV (coinfection; n = 7; red right bar) as determined by multiplexing to Mtb soluble cell wall, cell wall fraction, cell membrane, cytosol fraction, TX114 proteins, peptidoglycan and cell lysate. Change in median fluorescence intensity data was normalised via z ‐scoring before being represented as a heatmap (red indicates higher levels and blue indicates lower levels). Multiplex assays were repeated in duplicate.
Article Snippet: Mtb antigens of the H37Rv strain (sourced from
Techniques: Virus, Infection, Clinical Proteomics, Multiplexing, Membrane, Fluorescence, Multiplex Assay